PrimePCR™ Probe Assay: AHRR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: AHRR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AHRR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057513
Assay Design:   exonic
Chromosome Location:   5:353878-354021question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000413998 ENST00000372470 ENST00000505113 ENST00000316418 ENST00000514523 ENST00000510400 ENST00000515206 ENST00000504625 ENST00000319098 ENST00000568278 ENST00000569359 ENST00000567174 ENST00000566229 ENST00000261623

Gene Information

The protein encoded by this gene participates in the aryl hydrocarbon receptor (AhR) signaling cascade which mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. It functions as a feedback modulator by repressing AhR-dependent gene expression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jun 2011]

Gene Symbol:   AHRR
Gene Name:   aryl-hydrocarbon receptor repressor
Aliases:   AHH, AHHR, KIAA1234, MGC167813, MGC176630, bHLHe77
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000063438
Entrez:   57491
Chromosome Mapping:   5p15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.510000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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