PrimePCR™ Probe Assay: NAALADL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: NAALADL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NAALADL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057548
Assay Design:   exonic
Chromosome Location:   11:64815516-64815792question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000531220 ENST00000258704 ENST00000358658 ENST00000526799 ENST00000340252 ENST00000355721 ENST00000356632 ENST00000530995 ENST00000533340 ENST00000528884 ENST00000355369 ENST00000339885 ENST00000542096 ENST00000322396 ENST00000541232

Gene Information

Description Not Available

Gene Symbol:   NAALADL1
Gene Name:   N-acetylated alpha-linked acidic dipeptidase-like 1
Aliases:   I100, NAALADASEL
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000168060
Entrez:   10004
Chromosome Mapping:   11q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.230000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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