PrimePCR™ Probe Assay: RPL13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RPL13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPL13, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057697
Assay Design:   exonic
Chromosome Location:   16:89630235-89630378question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000311528 ENST00000452368 ENST00000393099

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq Jul 2011]

Gene Symbol:   RPL13
Gene Name:   ribosomal protein L13
Aliases:   BBC1, D16S444E, FLJ27453, FLJ27454, L13, MGC117342, MGC71373
RefSeq:   NC_000016.9 NT_010542.15
Ensembl:   ENSG00000167526
Entrez:   6137
Chromosome Mapping:   16q24.3,17p11.2

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