PrimePCR™ Probe Assay: VAMP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VAMP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VAMP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057927
Assay Design:   exonic
Chromosome Location:   17:8063230-8063352question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000316509

Gene Information

The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs syntaxins and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin synaptosomal-associated protein 25 kD and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq Jul 2008]

Gene Symbol:   VAMP2
Gene Name:   vesicle-associated membrane protein 2 (synaptobrevin 2)
Aliases:   FLJ11460, SYB2, VAMP-2
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000220205
Entrez:   6844
Chromosome Mapping:   17p13.1

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