PrimePCR™ Probe Assay: TRO, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TRO, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRO, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057945
Assay Design:   exonic
Chromosome Location:   X:54957693-54957823question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000173898 ENST00000319167 ENST00000375022 ENST00000399736 ENST00000375041 ENST00000420798

Gene Information

This gene encodes a membrane protein that mediates apical cell adhesion between trophoblastic cells and luminal epithelial cells of the endometrium and is implicated in the initial attachment during the process of embryo implantation. This gene is related to the MAGED gene family by sequence similarity and chromosome location. Multiple alternatively spliced transcript variants have been found for this gene; however the full-length nature of some variants has not been defined. [provided by RefSeq Jul 2008]

Gene Symbol:   TRO
Gene Name:   trophinin
Aliases:   KIAA1114, MAGE-d3, MAGED3
RefSeq:   NC_000023.10 NG_012806.1 NG_013254.1 NT_011630.14
Ensembl:   ENSG00000067445
Entrez:   7216
Chromosome Mapping:   Xp11.22-p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.470000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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