PrimePCR™ Probe Assay: POLR2J3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POLR2J3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: POLR2J3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057979
Assay Design:   exonic
Chromosome Location:   7:102181747-102182058question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000545661 ENST00000334583 ENST00000375248 ENST00000579208 ENST00000314256 ENST00000226091 ENST00000340457 ENST00000404971 ENST00000406316 ENST00000405472 ENST00000401669 ENST00000405581 ENST00000402717 ENST00000406859 ENST00000337018 ENST00000452918 ENST00000601879 ENST00000600377 ENST00000361677 ENST00000259253 ENST00000375990 ENST00000367742 ENST00000338920 ENST00000392078 ENST00000426496 ENST00000268171 ENST00000470056 ENST00000394104 ENST00000265956 ENST00000380871 ENST00000247226 ENST

Gene Information

This gene is a member of the RNA polymerase II subunit 11 gene family which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family DNA directed RNA polymerase II polypeptide J has been shown to encode a subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq Jul 2008]

Gene Symbol:   POLR2J3
Gene Name:   polymerase (RNA) II (DNA directed) polypeptide J3
Aliases:   MGC54043, POLR2J2, RPB11b1, RPB11b2
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000168255
Entrez:   548644
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.960000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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