PrimePCR™ Probe Assay: EEF1D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: EEF1D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EEF1D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057981
Assay Design:   exonic
Chromosome Location:   8:144662816-144663248question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000530109 ENST00000532741 ENST00000526838 ENST00000442189 ENST00000528610 ENST00000395119 ENST00000529272 ENST00000531621 ENST00000524624 ENST00000534380 ENST00000534377 ENST00000533204 ENST00000530191 ENST00000531218 ENST00000533494 ENST00000530445 ENST00000529516 ENST00000423316 ENST00000419152 ENST00000317198

Gene Information

This gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit delta functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1 6 7 9 11 13 17 19.[provided by RefSeq Aug 2010]

Gene Symbol:   EEF1D
Gene Name:   eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Aliases:   EF-1D, EF1D, FLJ20897, FP1047
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000104529
Entrez:   1936
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.810000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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