PrimePCR™ Probe Assay: FBXW10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: FBXW10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FBXW10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058020
Assay Design:   exonic
Chromosome Location:   17:18647366-18647511question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000395665 ENST00000308799 ENST00000301938 ENST00000395667

Gene Information

Members of the F-box protein family such as FBXW10 are characterized by an approximately 40-amino acid F-box motif. SCF complexes formed by SKP1 (MIM 601434) cullin (see CUL1; MIM 603134) and F-box proteins act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box and they interact with ubiquitination targets through other protein interaction domains (Jin et al. 2004 [PubMed 15520277]).[supplied by OMIM Mar 2008]

Gene Symbol:   FBXW10
Gene Name:   F-box and WD repeat domain containing 10
Aliases:   Fbw10, HREP, SM25H2, SM2SH2
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000171931
Entrez:   10517
Chromosome Mapping:   17p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.640000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download