PrimePCR™ Probe Assay: SSH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SSH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SSH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058061
Assay Design:   exonic
Chromosome Location:   12:109194579-109194692question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000326495 ENST00000551165 ENST00000326470 ENST00000360239

Gene Information

The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1) which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   SSH1
Gene Name:   slingshot homolog 1 (Drosophila)
Aliases:   FLJ21928, FLJ38102, KIAA1298, SSH1L
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000084112
Entrez:   54434
Chromosome Mapping:   12q24.11

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