PrimePCR™ Probe Assay: CD59, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CD59, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CD59, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058252
Assay Design:   exonic
Chromosome Location:   11:33731741-33731841question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000534312 ENST00000395850 ENST00000533403 ENST00000351554 ENST00000415002 ENST00000445143 ENST00000426650 ENST00000437761 ENST00000527577 ENST00000528700

Gene Information

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex whereby it binds complement C8 and/or C9 during the assembly of this complex thereby inhibiting the incorporation of multiple copies of C9 into the complex which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency a disease resulting in hemolytic anemia and thrombosis and which causes cerebral infarction. Multiple alternatively spliced transcript variants which encode the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CD59
Gene Name:   CD59 molecule, complement regulatory protein
Aliases:   16.3A5, 1F5, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20
RefSeq:   NC_000011.9 NT_009237.18 NG_008057.1
Ensembl:   ENSG00000085063
Entrez:   966
Chromosome Mapping:   11p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.450000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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