PrimePCR™ Probe Assay: INSL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: INSL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: INSL3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058334
Assay Design:   exonic
Chromosome Location:   19:17927450-17927562question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000317306 ENST00000598577 ENST00000379695

Gene Information

The protein encoded by this gene is an insulin like hormone produced mainly in gonadal tissues in males and females. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. It may also act as a hormone to regulate growth and differentiation of gubernaculum and thus mediating intra-abdominal testicular descent. The mutations in this gene may lead to but not a frequent cause of cryptorchidism. [provided by RefSeq Jul 2008]

Gene Symbol:   INSL3
Gene Name:   insulin-like 3 (Leydig cell)
Aliases:   MGC119818, MGC119819, RLF, RLNL
RefSeq:   NC_000019.9 NG_012092.1 NT_011295.11
Ensembl:   ENSG00000248099
Entrez:   3640
Chromosome Mapping:   19p13.2-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.440000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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