PrimePCR™ Probe Assay: PLA2G7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058366
Assay Design:   exonic
Chromosome Location:   6:46678389-46679320question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000274793 ENST00000537365 ENST00000538237 ENST00000541026

Gene Information

The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq Dec 2009]

Gene Symbol:   PLA2G7
Gene Name:   phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Aliases:   LDL-PLA2, LP-PLA2, PAFAH
RefSeq:   NC_000006.11 NG_016204.1 NT_007592.15
Ensembl:   ENSG00000146070
Entrez:   7941
Chromosome Mapping:   6p21.2-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.070000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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