PrimePCR™ Probe Assay: ECE2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ECE2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ECE2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058380
Assay Design:   exonic
Chromosome Location:   3:184001673-184002759question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000402825 ENST00000359140 ENST00000404464 ENST00000357474 ENST00000430587

Gene Information

Endothelin-converting enzymes such as ECE2 (EC 3.4.24.71) are type II metalloproteases that generate functionally pleiotropic members of the endothelin vasoactive peptide family (Lorenzo et al. 2001 [PubMed 11718899]).[supplied by OMIM Mar 2008]

Gene Symbol:   ECE2
Gene Name:   endothelin converting enzyme 2
Aliases:   KIAA0604, MGC17664, MGC2408, MGC78487
RefSeq:   NC_000003.11 NT_005612.16 NG_008924.2
Ensembl:   ENSG00000145194
Entrez:   9718
Chromosome Mapping:   3q27.1,.

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.570000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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