PrimePCR™ Probe Assay: CYP1A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP1A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP1A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058439
Assay Design:   exonic
Chromosome Location:   15:75014745-75014837question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000379727 ENST00000567032 ENST00000395049 ENST00000564596 ENST00000566503 ENST00000395048

Gene Information

This gene CYP1A1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs) some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member CYP1A2 is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP1A1
Gene Name:   cytochrome P450, family 1, subfamily A, polypeptide 1
Aliases:   AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX
RefSeq:   NC_000015.9 NG_008431.1 NT_010194.17
Ensembl:   ENSG00000140465
Entrez:   1543
Chromosome Mapping:   15q24.1

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