PrimePCR™ Probe Assay: DNM1L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DNM1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DNM1L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058457
Assay Design:   exonic
Chromosome Location:   12:32832300-32854357question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000452533 ENST00000553257 ENST00000549701 ENST00000358214 ENST00000266481 ENST00000551476 ENST00000550154 ENST00000547312 ENST00000381000 ENST00000548750 ENST00000414834

Gene Information

The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily including the S. cerevisiae proteins Dnm1 and Vps1 contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq Jul 2008]

Gene Symbol:   DNM1L
Gene Name:   dynamin 1-like
Aliases:   DLP1, DRP1, DVLP, DYMPLE, DYNIV-11, FLJ41912, HDYNIV, VPS1
RefSeq:   NC_000012.11 NG_028122.1 NT_009714.17 NG_012219.1
Ensembl:   ENSG00000087470
Entrez:   10059
Chromosome Mapping:   12p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.980000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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