PrimePCR™ SYBR® Green Assay: DMD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027701

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DMD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DMD, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010707
Assay Design:   Intron-spanning
Chromosome Location:   X:31200991-31222205question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000378723 ENST00000358062 ENST00000378677 ENST00000357033 ENST00000378702 ENST00000465285 ENST00000474231 ENST00000361471 ENST00000378680 ENST00000378705 ENST00000534884 ENST00000378682 ENST00000378684 ENST00000359836 ENST00000343523 ENST00000542849 ENST00000535280 ENST00000378707 ENST00000541735

Gene Information

The dystrophin gene is the largest gene found in nature measuring 2.4 Mb. The gene was identified through a positional cloning approach targeted at the isolation of the gene responsible for Duchenne (DMD) and Becker (BMD) Muscular Dystrophies. DMD is a recessive fatal X-linked disorder occurring at a frequency of about 1 in 3500 new-born males. BMD is a milder allelic form. In general DMD patients carry mutations which cause premature translation termination (nonsense or frame shift mutations) while in BMD patients dystrophin is reduced either in molecular weight (derived from in-frame deletions) or in expression level. The dystrophin gene is highly complex containing at least eight independent tissue-specific promoters and two polyA-addition sites. Furthermore dystrophin RNA is differentially spliced producing a range of different transcripts encoding a large set of protein isoforms. Dystrophin (as encoded by the Dp427 transcripts) is a large rod-like cytoskeletal protein which is found at the inner surface of muscle fibers. Dystrophin is part of the dystrophin-glycoprotein complex (DGC) which bridges the inner cytoskeleton (F-actin) and the extra-cellular matrix. [provided by RefSeq Jul 2008]

Gene Symbol:   DMD
Gene Name:   dystrophin
Aliases:   BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272
RefSeq:   NC_000023.10 NT_167197.1 NG_012232.1
Ensembl:   ENSG00000198947
Entrez:   1756
Chromosome Mapping:   Xp21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download