Gene:  DMD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The dystrophin gene is the largest gene found in nature measuring 2.4 Mb. The gene was identified through a positional cloning approach targeted at the isolation of the gene responsible for Duchenne (DMD) and Becker (BMD) Muscular Dystrophies. DMD is a recessive fatal X-linked disorder occurring at a frequency of about 1 in 3500 new-born males. BMD is a milder allelic form. In general DMD patients carry mutations which cause premature translation termination (nonsense or frame shift mutations) while in BMD patients dystrophin is reduced either in molecular weight (derived from in-frame deletions) or in expression level. The dystrophin gene is highly complex containing at least eight independent tissue-specific promoters and two polyA-addition sites. Furthermore dystrophin RNA is differentially spliced producing a range of different transcripts encoding a large set of protein isoforms. Dystrophin (as encoded by the Dp427 transcripts) is a large rod-like cytoskeletal protein which is found at the inner surface of muscle fibers. Dystrophin is part of the dystrophin-glycoprotein complex (DGC) which bridges the inner cytoskeleton (F-actin) and the extra-cellular matrix. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: DMD, Human
dystrophin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010707
Info:   Same primer pair as used in probe assay qHsaCIP0027701
List Price:    $146.00
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PrimePCR™ Probe Assay: DMD, Human
dystrophin

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027701
List Price:    $214.00
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