PrimePCR™ Probe Assay: ITGAM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ITGAM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ITGAM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0025989
Assay Design:   Intron-spanning
Chromosome Location:   16:31332809-31335755question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000544665 ENST00000287497

Gene Information

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1') or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   ITGAM
Gene Name:   integrin, alpha M (complement component 3 receptor 3 subunit)
Aliases:   CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6
RefSeq:   NC_000016.9 NT_010393.16 NG_011719.1
Ensembl:   ENSG00000169896
Entrez:   3684
Chromosome Mapping:   16p11.2

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