PrimePCR™ Probe Assay: MTNR1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MTNR1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MTNR1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0025997
Assay Design:   Intron-spanning
Chromosome Location:   4:187455568-187476367question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000307161

Gene Information

This gene encodes one of two high affinity forms of a receptor for melatonin the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq Jul 2008]

Gene Symbol:   MTNR1A
Gene Name:   melatonin receptor 1A
Aliases:   MEL-1A-R, MT1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000168412
Entrez:   4543
Chromosome Mapping:   4q35.1

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