PrimePCR™ Probe Assay: OSBPL1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026071
Assay Design:   Intron-spanning
Chromosome Location:   18:21805110-21819234question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000319481 ENST00000399443 ENST00000357041

Gene Information

This gene encodes a member of the oxysterol-binding protein (OSBP) family a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   OSBPL1A
Gene Name:   oxysterol binding protein-like 1A
Aliases:   FLJ10217, ORP-1, ORP1, OSBPL1B
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000141447
Entrez:   114876
Chromosome Mapping:   18q11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.270000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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