PrimePCR™ Probe Assay: SLC35D2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: SLC35D2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC35D2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026098
Assay Design:   Intron-spanning
Chromosome Location:   9:99126766-99145805question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000253270 ENST00000375257 ENST00000375259

Gene Information

Nucleotide sugars which are synthesized in the cytosol or the nucleus are high-energy donor substrates for glycosyltransferases located in the lumen of the endoplasmic reticulum and Golgi apparatus. Translocation of nucleotide sugars from the cytosol into the lumen compartment is mediated by specific nucleotide sugar transporters such as SLC35D2 (Suda et al. 2004 [PubMed 15082721]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC35D2
Gene Name:   solute carrier family 35, member D2
Aliases:   HFRC1, MGC117215, MGC142139, SQV7L, UGTrel8, hfrc
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000130958
Entrez:   11046
Chromosome Mapping:   9q22.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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