PrimePCR™ Probe Assay: CLCN5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CLCN5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLCN5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026407
Assay Design:   Intron-spanning
Chromosome Location:   X:49855443-49856863question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000376088 ENST00000376091 ENST00000376108 ENST00000307367 ENST00000450422

Gene Information

This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.]

Gene Symbol:   CLCN5
Gene Name:   chloride channel 5
Aliases:   CLC5, CLCK2, DENTS, NPHL1, NPHL2, XLRH, XRN, hCIC-K2, hClC-K2
RefSeq:   NC_000023.10 NG_007159.2 NT_086939.3
Ensembl:   ENSG00000171365
Entrez:   1184
Chromosome Mapping:   Xp11.23-p11.22

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