PrimePCR™ Probe Assay: DGKG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DGKG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DGKG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026543
Assay Design:   Intron-spanning
Chromosome Location:   3:185990105-185993424question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000265022 ENST00000344484 ENST00000382164 ENST00000437018 ENST00000544847 ENST00000538691

Gene Information

This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol a fundamental lipid second messenger that activates numerous proteins including protein kinase C isoforms Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DGKG
Gene Name:   diacylglycerol kinase, gamma 90kDa
Aliases:   DAGK3, DGK-GAMMA, MGC104993, MGC133330
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000058866
Entrez:   1608
Chromosome Mapping:   3q27.2-q27.3

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