PrimePCR™ Probe Assay: GUCY2F, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GUCY2F, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GUCY2F, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026548
Assay Design:   Intron-spanning
Chromosome Location:   X:108719167-108725186question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000218006

Gene Information

The protein encoded by this gene is a guanylyl cyclase found predominantly in photoreceptors in the retina. The encoded protein is thought to be involved in resynthesis of cGMP after light activation of the visual signal transduction cascade allowing a return to the dark state. This protein is a single-pass type I membrane protein. Defects in this gene may be a cause of X-linked retinitis pigmentosa. [provided by RefSeq Dec 2008]

Gene Symbol:   GUCY2F
Gene Name:   guanylate cyclase 2F, retinal
Aliases:   CYGF, GC-F, GUC2DL, GUC2F, RETGC-2, ROS-GC2
RefSeq:   NC_000023.10 NG_009175.1 NT_011651.17
Ensembl:   ENSG00000101890
Entrez:   2986
Chromosome Mapping:   Xq22

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