PrimePCR™ Probe Assay: POU1F1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POU1F1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POU1F1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026559
Assay Design:   Intron-spanning
Chromosome Location:   3:87311350-87313546question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000350375 ENST00000344265

Gene Information

This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   POU1F1
Gene Name:   POU class 1 homeobox 1
Aliases:   CPHD1, GHF-1, PIT1, POU1F1a, Pit-1
RefSeq:   NC_000003.11 NT_022459.15 NG_008225.1
Ensembl:   ENSG00000064835
Entrez:   5449
Chromosome Mapping:   3p11

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