PrimePCR™ Probe Assay: ATP6V1C2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP6V1C2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP6V1C2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026744
Assay Design:   Intron-spanning
Chromosome Location:   2:10918732-10922453question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000272238 ENST00000381661

Gene Information

This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three Athree B and two G subunits as well as a C D E F and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants encoding different V1 domain C subunit isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP6V1C2
Gene Name:   ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2
Aliases:   ATP6C2, VMA5
RefSeq:   NC_000002.11 NT_005334.16
Ensembl:   ENSG00000143882
Entrez:   245973
Chromosome Mapping:   -

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