PrimePCR™ Probe Assay: DYNC1LI2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: DYNC1LI2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DYNC1LI2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026829
Assay Design:   Intron-spanning
Chromosome Location:   16:66766304-66769981question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000258198 ENST00000443351 ENST00000440564

Gene Information

Cytoplasmic dynein is a microtubule-associated motor protein (Hughes et al. 1995 [PubMed 7738094]). See DYNC1H1 (MIM 600112) for general information about dyneins.[supplied by OMIM Mar 2008]

Gene Symbol:   DYNC1LI2
Gene Name:   dynein, cytoplasmic 1, light intermediate chain 2
Aliases:   DNCLI2, LIC2
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000135720
Entrez:   1783
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.400000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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