PrimePCR™ Probe Assay: MED30, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MED30, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MED30, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026960
Assay Design:   Intron-spanning
Chromosome Location:   8:118533240-118540947question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000297347 ENST00000522839

Gene Information

The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al. 2002 [PubMed 11909976]).[supplied by OMIM Nov 2010]

Gene Symbol:   MED30
Gene Name:   mediator complex subunit 30
Aliases:   MED30S, MGC9890, THRAP6, TRAP25
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000164758
Entrez:   90390
Chromosome Mapping:   8q24.11

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