PrimePCR™ Probe Assay: NDUFS2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NDUFS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NDUFS2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026974
Assay Design:   Intron-spanning
Chromosome Location:   1:161176318-161179066question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000367993 ENST00000392179 ENST00000476409

Gene Information

The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits 7 of which are encoded by the mitochondrial genome and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   NDUFS2
Gene Name:   NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Aliases:   CI-49
RefSeq:   NC_000001.10 NT_004487.19 NG_013352.1
Ensembl:   ENSG00000158864
Entrez:   4720
Chromosome Mapping:   1q23

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