PrimePCR™ Probe Assay: NR2C2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NR2C2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NR2C2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027012
Assay Design:   Intron-spanning
Chromosome Location:   3:15070139-15071831question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000425241 ENST00000323373 ENST00000393102 ENST00000406272

Gene Information

Members of the nuclear hormone receptor family such as NR2C2 act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain a central DNA-binding domain with 2 zinc fingers and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al. 1996 [PubMed 8661150]).[supplied by OMIM Mar 2008]

Gene Symbol:   NR2C2
Gene Name:   nuclear receptor subfamily 2, group C, member 2
Aliases:   TAK1, TR2R1, TR4, hTAK1
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000177463
Entrez:   7182
Chromosome Mapping:   3p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.240000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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