PrimePCR™ Probe Assay: LRSAM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LRSAM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LRSAM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027051
Assay Design:   Intron-spanning
Chromosome Location:   9:130243479-130248064question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000373324 ENST00000323301 ENST00000373322 ENST00000300417

Gene Information

This gene encodes a ring finger protein involved in a variety of functions including regulation of signaling pathways and cell adhesion mediation of self-ubiquitylation and involvement in cargo sorting during receptor endocytosis. Mutations in this gene have been associated with Charcot-Marie-Tooth disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   LRSAM1
Gene Name:   leucine rich repeat and sterile alpha motif containing 1
Aliases:   FLJ31641, RIFLE, TAL
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000148356
Entrez:   90678
Chromosome Mapping:   9q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.500000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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