PrimePCR™ Probe Assay: CCDC88A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CCDC88A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCDC88A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027053
Assay Design:   Intron-spanning
Chromosome Location:   2:55544917-55546085question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000336838 ENST00000263630 ENST00000412148 ENST00000426576 ENST00000456975 ENST00000436346 ENST00000413716

Gene Information

This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   CCDC88A
Gene Name:   coiled-coil domain containing 88A
Aliases:   APE, DKFZp686D0630, GIRDIN, GIV, GRDN, HkRP1, KIAA1212
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115355
Entrez:   55704
Chromosome Mapping:   2p16.1

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