PrimePCR™ Probe Assay: RBFOX2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RBFOX2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RBFOX2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027070
Assay Design:   Intron-spanning
Chromosome Location:   22:36157462-36164313question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000405409 ENST00000414461 ENST00000449924 ENST00000262829 ENST00000397303 ENST00000359369 ENST00000416721 ENST00000438146 ENST00000338644

Gene Information

This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RBFOX2
Gene Name:   RNA binding protein, fox-1 homolog (C. elegans) 2
Aliases:   FOX2, Fox-2, HNRBP2, HRNBP2, RBM9, RTA, dJ106I20.3, fxh
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100320
Entrez:   23543
Chromosome Mapping:   22q13.1

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