PrimePCR™ Probe Assay: SEC16A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SEC16A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEC16A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027095
Assay Design:   Intron-spanning
Chromosome Location:   9:139355688-139357410question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000277537 ENST00000453963 ENST00000371706 ENST00000313050 ENST00000290037 ENST00000431893 ENST00000404925

Gene Information

The selective export of proteins and lipids from the endoplasmic reticulum (ER) is mediated by the coat protein complex II (COPII) that assembles at discrete sites on the membrane known as endoplasmic reticulum exit sites (ERES) or transitional ER sites. SEC16A is a peripheral membrane protein that defines ERES in mammalian cells and is required for protein transport from ER to Golgi (Watson et al. 2006 [PubMed 17005010]).[supplied by OMIM Jun 2009]

Gene Symbol:   SEC16A
Gene Name:   SEC16 homolog A (S. cerevisiae)
Aliases:   FLJ26737, KIAA0310, RP11-413M3.10, SEC16L, p250
RefSeq:   NC_000009.11 NG_016126.1 NT_024000.16
Ensembl:   ENSG00000148396
Entrez:   9919
Chromosome Mapping:   9q34.3

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