PrimePCR™ Probe Assay: SPTB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SPTB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPTB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027124
Assay Design:   Intron-spanning
Chromosome Location:   14:65251061-65252494question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000556626 ENST00000389721 ENST00000389723 ENST00000389722 ENST00000335612 ENST00000542895 ENST00000389720

Gene Information

This locus encodes a member of the spectrin gene family. Spectrin proteins along with ankyrin play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes and mutations in this gene have been associated with spherocytosis type 2 hereditary elliptocytosis and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq Nov 2009]

Gene Symbol:   SPTB
Gene Name:   spectrin, beta, erythrocytic
Aliases:   EL3, HS2, HSPTB1, SPH2
RefSeq:   NC_000014.8 NT_026437.12 NG_016202.1
Ensembl:   ENSG00000070182
Entrez:   6710
Chromosome Mapping:   14q23-q24.2

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