PrimePCR™ Probe Assay: PNPO, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PNPO, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PNPO, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027238
Assay Design:   Intron-spanning
Chromosome Location:   17:46020707-46022068question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000225573 ENST00000434554 ENST00000544840

Gene Information

The enzyme encoded by this gene catalyzes the terminal rate-limiting step in the synthesis of pyridoxal 5'-phosphate also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency a form of neonatal epileptic encephalopathy. [provided by RefSeq Oct 2008]

Gene Symbol:   PNPO
Gene Name:   pyridoxamine 5'-phosphate oxidase
Aliases:   FLJ10535, PDXPO
RefSeq:   NC_000017.10 NG_008744.1 NT_010783.15
Ensembl:   ENSG00000108439
Entrez:   55163
Chromosome Mapping:   17q21.32

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