PrimePCR™ Probe Assay: GTF2A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GTF2A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GTF2A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027258
Assay Design:   Intron-spanning
Chromosome Location:   14:81659120-81662544question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000553612 ENST00000434192 ENST00000344860

Gene Information

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder 1993 [PubMed 8224848]). One of these factors is TFIIA which when purified from HeLa extracts consists of 35- 19- and 12-kD subunits.[supplied by OMIM Jul 2010]

Gene Symbol:   GTF2A1
Gene Name:   general transcription factor IIA, 1, 19/37kDa
Aliases:   MGC129969, MGC129970, TF2A1, TFIIA
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000165417
Entrez:   2957
Chromosome Mapping:   14q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.850000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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