PrimePCR™ Probe Assay: KCND3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KCND3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KCND3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027337
Assay Design:   Intron-spanning
Chromosome Location:   1:112525377-112531477question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000315987 ENST00000302127

Gene Information

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release heart rate insulin secretion neuronal excitability epithelial electrolyte transport smooth muscle contraction and cell volume. Four sequence-related potassium channel genes - shaker shaw shab and shal - have been identified in Drosophila and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel voltage-gated shal-related subfamily members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes which are encoded by alternatively spliced transcript variants of this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KCND3
Gene Name:   potassium voltage-gated channel, Shal-related subfamily, member 3
Aliases:   KCND3L, KCND3S, KSHIVB, KV4.3, MGC142035, MGC142037
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000171385
Entrez:   3752
Chromosome Mapping:   1p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.930000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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