PrimePCR™ Probe Assay: NPFFR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NPFFR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NPFFR2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027411
Assay Design:   Intron-spanning
Chromosome Location:   4:72994505-73003802question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000308744 ENST00000395999 ENST00000358749

Gene Information

This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2009]

Gene Symbol:   NPFFR2
Gene Name:   neuropeptide FF receptor 2
Aliases:   GPR74, HLWAR77, NPFF2, NPGPR
RefSeq:   NC_000004.11 NT_022778.16
Ensembl:   ENSG00000056291
Entrez:   10886
Chromosome Mapping:   4q21

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