PrimePCR™ Probe Assay: PANK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PANK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PANK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027497
Assay Design:   Intron-spanning
Chromosome Location:   10:91348465-91353026question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000342512 ENST00000322191 ENST00000307534 ENST00000371775 ENST00000371774

Gene Information

This gene encodes a member of the pantothenate kinase family. Pantothenate kinases are key regulatory enzymes in the biosynthesis of coenzyme A (CoA). The encoded protein catalyzes the first and rate-limiting enzymatic reaction in CoA biosynthesis and is regulated by CoA through feedback inhibition. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene and an intronic miRNA on the same strand are co-regulated by the tumor suppressor p53 (see PMID 20833636). [provided by RefSeq Apr 2011]

Gene Symbol:   PANK1
Gene Name:   pantothenate kinase 1
Aliases:   MGC24596, PANK
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000152782
Entrez:   53354
Chromosome Mapping:   10q23.31

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