PrimePCR™ Probe Assay: MAPKAP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MAPKAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAPKAP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027562
Assay Design:   Intron-spanning
Chromosome Location:   9:128322052-128347905question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000373511 ENST00000350766 ENST00000373503 ENST00000373498 ENST00000420643 ENST00000394060 ENST00000427078 ENST00000265960 ENST00000394063 ENST00000373505

Gene Information

This gene encodes a protein that is highly similar to the yeast SIN1 protein a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPKAP1
Gene Name:   mitogen-activated protein kinase associated protein 1
Aliases:   JC310, MGC2745, MIP1, SIN1, SIN1b, SIN1g
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000119487
Entrez:   79109
Chromosome Mapping:   9q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 33.800000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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