PrimePCR™ Probe Assay: FXYD5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FXYD5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FXYD5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027598
Assay Design:   Intron-spanning
Chromosome Location:   19:35646454-35648375question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000392218 ENST00000392219 ENST00000342879 ENST00000541435 ENST00000423817 ENST00000543307

Gene Information

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2 also known as the gamma subunit of the NaK-ATPase regulates the properties of that enzyme. FXYD1 (phospholemman) FXYD2 (gamma) FXYD3 (MAT-8) FXYD4 (CHIF) and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2) with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product FXYD5 is a glycoprotein that functions in the up-regulation of chemokine production and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants. [RefSeq curation by Kathleen J. Sweadner Ph.D. sweadner@helix.mgh.harvard.edu. Sep 2009]

Gene Symbol:   FXYD5
Gene Name:   FXYD domain containing ion transport regulator 5
Aliases:   DYSAD, IWU1, KCT1, OIT2, PRO6241, RIC
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000089327
Entrez:   53827
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 33.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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