PrimePCR™ Probe Assay: MYL5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYL5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYL5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027631
Assay Design:   Intron-spanning
Chromosome Location:   4:672770-674315question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000506838 ENST00000505477 ENST00000511290 ENST00000400159 ENST00000507804

Gene Information

This gene encodes one of the myosin light chains a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains two nonphosphorylatable alkali light chains and two phosphorylatable regulatory light chains. This gene product one of the regulatory light chains is expressed in fetal muscle and in adult retina cerebellum and basal ganglia. [provided by RefSeq Jul 2008]

Gene Symbol:   MYL5
Gene Name:   myosin, light chain 5, regulatory
Aliases:   Not Available
RefSeq:   NC_000004.11 NT_037622.5
Ensembl:   ENSG00000215375
Entrez:   4636
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.530000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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