PrimePCR™ Probe Assay: GPR161, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GPR161, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GPR161, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027685
Assay Design:   Intron-spanning
Chromosome Location:   1:168054970-168056886question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000367838 ENST00000367836 ENST00000367835 ENST00000546300 ENST00000539777 ENST00000537209 ENST00000271357 ENST00000361697

Gene Information

Upon ligand binding G protein-coupled receptors such as GPR161 activate cytoplasmic G proteins (see GNAS MIM 139320) allowing the receptors to transduce extracellular signals across the plasma membrane into the cell. Phosphorylation of the receptor attenuates signaling (Matteson et al. 2008 [PubMed 18250320]).[supplied by OMIM Aug 2008]

Gene Symbol:   GPR161
Gene Name:   G protein-coupled receptor 161
Aliases:   FLJ33952, RE2
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143147
Entrez:   23432
Chromosome Mapping:   1q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.310000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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