PrimePCR™ Probe Assay: IGF2BP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IGF2BP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: IGF2BP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027716
Assay Design:   Intron-spanning
Chromosome Location:   17:47118807-47119659question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000290341 ENST00000431824

Gene Information

This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes including insulin-like growth factor 2 beta-actin and beta-transducin repeat-containing protein and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2009]

Gene Symbol:   IGF2BP1
Gene Name:   insulin-like growth factor 2 mRNA binding protein 1
Aliases:   CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000159217
Entrez:   10642
Chromosome Mapping:   17q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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