PrimePCR™ Probe Assay: DDAH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DDAH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DDAH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027727
Assay Design:   Intron-spanning
Chromosome Location:   1:85787213-85790554question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000284031 ENST00000539042 ENST00000426972 ENST00000542148 ENST00000535924

Gene Information

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines which in turn inhibit nitric oxide synthase activity. [provided by RefSeq Jul 2008]

Gene Symbol:   DDAH1
Gene Name:   dimethylarginine dimethylaminohydrolase 1
Aliases:   DDAH, FLJ21264, FLJ25539
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000153904
Entrez:   23576
Chromosome Mapping:   1p22

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