PrimePCR™ Probe Assay: KDM5C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KDM5C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KDM5C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027745
Assay Design:   Intron-spanning
Chromosome Location:   X:53243913-53245024question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000375401 ENST00000404049 ENST00000375379 ENST00000375383 ENST00000452825

Gene Information

This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain one JmjC domain one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2009]

Gene Symbol:   KDM5C
Gene Name:   lysine (K)-specific demethylase 5C
Aliases:   DXS1272E, JARID1C, MRXJ, MRXSJ, SMCX, XE169
RefSeq:   NC_000023.10 NT_011630.14 NG_008085.1
Ensembl:   ENSG00000126012
Entrez:   8242
Chromosome Mapping:   Xp11.22-p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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