PrimePCR™ Probe Assay: CACNA1G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CACNA1G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CACNA1G, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027848
Assay Design:   Intron-spanning
Chromosome Location:   17:48681577-48683341question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000502264 ENST00000512389 ENST00000513964 ENST00000514717 ENST00000510366 ENST00000503485 ENST00000507510 ENST00000507336 ENST00000513689 ENST00000507609 ENST00000510115 ENST00000515165 ENST00000514181 ENST00000515765 ENST00000514079 ENST00000352832 ENST00000442258 ENST00000360761 ENST00000416767 ENST00000354983 ENST00000506520 ENST00000358244 ENST00000359106 ENST00000429973 ENST00000515411 ENST00000505165 ENST00000507896

Gene Information

Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes including muscle contraction hormone or neurotransmitter release gene expression cell motility cell division and cell death. This gene encodes a T-type low-voltage activated calcium channel. The T-type channels generate currents that are both transient owing to fast inactivation and tiny owing to small conductance. T-type channels are thought to be involved in pacemaker activity low-threshold calcium spikes neuronal oscillations and resonance and rebound burst firing. Many alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   CACNA1G
Gene Name:   calcium channel, voltage-dependent, T type, alpha 1G subunit
Aliases:   Ca(V)T.1, Cav3.1, MGC117234, NBR13
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000006283
Entrez:   8913
Chromosome Mapping:   17q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.150000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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