PrimePCR™ Probe Assay: KRT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KRT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027943
Assay Design:   Intron-spanning
Chromosome Location:   12:53071483-53072372question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000252244

Gene Information

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT1
Gene Name:   keratin 1
Aliases:   CK1, EHK, EHK1, EPPK, K1, KRT1A, NEPPK
RefSeq:   NC_000012.11 NG_008364.1 NT_029419.12
Ensembl:   ENSG00000167768
Entrez:   3848
Chromosome Mapping:   12q13.13

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