PrimePCR™ Probe Assay: CYP26A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027952
Assay Design:   Intron-spanning
Chromosome Location:   10:94834933-94835624question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000371531 ENST00000224356 ENST00000394139

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This endoplasmic reticulum protein acts on retinoids including all-trans-retinoic acid (RA) with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene which encode the distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP26A1
Gene Name:   cytochrome P450, family 26, subfamily A, polypeptide 1
Aliases:   CP26, CYP26, P450RAI, P450RAI1
RefSeq:   NC_000010.10 NG_008067.1 NT_030059.13
Ensembl:   ENSG00000095596
Entrez:   1592
Chromosome Mapping:   10q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.370000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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